Year: 2014 | Month: December | Volume 4 | Issue 3

One / Two Days Preservation of goat oocytes at refrigerator and their maturation in vitro


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Abstract:

This study was conduct to evaluate the feasibility of one / two days preserving immature goat oocytes without freezing. Cumulus oocyte complexes (COCs) were obtained from ovaries of slaughtered goats. Selected COCs were kept in tissue culture medium (TCM-199) and 10% Fetal Calf Serum (FCS) and stored at 4°C for 1 or 2 days. After preservation, oocyte morphology and viability were evaluated. Post-warmed stored and Non-stored oocytes were cultured in TCM-199 and 10% FCS in CO2 incubator for 30 h. Oocyte maturation was indicated by cumulus expansion after in vitro culture. Data analysis revealed a high percentage of morphologically normal and viable oocytes after preservation either for 1 or 2 days (70, 60 vs. 60, 50%, respectively) with low percentage of damaged oocytes. There was no significant difference in the maturation of stored oocytes for 1 or 2 days in comparison to fresh oocytes although  the  value  of  the  fresh oocytes was higher (   55, 50 vs. 65%, respectively). Thus, we have developed a simple method for preserving immature goat oocytes by refrigeration at 4°C for two days without evident damage of oocyte and keeping oocyte developmental competence.





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