Year: 2014 | Month: December | Volume 4 | Issue 3

Effect of multiple vs. single preovulatory follicle on oocyte quantity and quality and in vitro maturation of goat oocytes


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Abstract:

The present study was conducted to investigate the effects of multiple vs. single preovulatory follicle on oocyte quantity and quality and in vitro maturation of goat oocytes. Slaughtered goat ovaries were collected and ranked into 2 types according to the number of preovulatory follicles on their surface: Type I (with multiple preovulatroy follicles, > 2 follicles), Type II (with single preovulatory follicle). The number of goat oocytes retrieved from follicles (2-6 mm Ø) was recorded for each ovarian type. The released immature goat oocytes were scored for cumulus - oocyte cell adhesion into one of 3 grades (C+; cumulus - enclosed oocytes, C+/-; cumulus partially enclosed oocytes, C- ; cumulus - free oocytes). Grade C+ and grade C+/ were matured in tissue culture media (TCM -199) supplemented with 10% Fetal Calf Serum for 30 h in CO2 incubator at 38.5ºC and 95% humidity. The results indicated that a greater number of aspirated oocytes were found in type I than type II. The number of Grade C+ and Grade C+/- oocytes in Type I was significantly higher (P < 0.05) than type II. The in vitro maturation rate of oocytes recovered from goat ovaries was non significantly different in both types but the number of cumulus - full expanded oocytes (CE+) appeared to be higher in ovaries having multiple than single preovulatory follicle. In conclusion, higher quantity, quality and maturation rate of oocytes may produce in multiple preovulatory follicles of goat ovaries.





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