Original Article

Year: 2020 | Month: August | Volume 10 | Issue 2

Endometrial Cytological Evaluations in Repeat Breeding Crossbred Cows

Anil Kumar Pandey Utsav Sharma Sharad Kumar
DOI:10.30954/2277-3371.02.2020.2

Aim:

Endometrial cytological study using cytobrush technique was conducted on 80 crossbred cows under field condition to evaluate the prevalence of subclinical endometritis. Animals were grouped in three groups, the group-I (n=20): normal cyclic cows having no previous repeat breeding history and had clear stringy vaginal mucus discharge on estrus; the group-II (n=40): repeat breeding cows, had clear stringy vaginal mucus discharge on estrus; and the group-III (n=20): endometritic cows, had turbid vaginal mucus with visible white flakes. The endometrial cell cytology was performed using modified cytobrush technique. All animals with clear stringy cervico-vaginal mucus discharge (group-I and group-II) were inseminated without any treatment. The mean polymorphonuclear (PMN) cell % in the normal cyclic, repeat breeding and endometritis group of cows were 2.01 ± 0.25, 5.36 ± 0.39, 11.82 ± 0.82 with 1(5%), 26(65%) and 20(100%) animals having >4% PMN cells in the respective group of cows. The overall conception rate in normal cyclic group was 95% after 3 inseminations while in the repeat breeding group it was 10%. Cows with turbid discharge (group-III) were given 20 ml liquid Levofloxacin + Ornidazole + α-Tocopherol (Lenovo AP; Intas Pharmaceuticals-vet) intra-uterine infusion for three consecutive days of the estrus and AI was performed on the next estrus, resulted conception of 35% animals in two inseminations. Cytobrush technique was observed useful in differentiating subclinical endometritis in the repeat breeding cows and Levofloxacin + Ornidazole + α-Tocopherol (Lenovo AP) treatment was satisfactory in the cows showing turbid mucus discharge.



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